Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/04/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab Cloning Bootcamp</span>
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Daniel Vargas - 04/29/2015==
mCherry pSB1A3 using Amp / DH5α-Turbo
Inoculation 04/28/15 http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp/2015/04/28
Liquid culture 1: growth
Liquid culture 2: growth
Streak 1: growth
Streak 2: growth
'''Minipreps'''
Sample 1: mCherry pSB1A3 colony 1; 2H5Q; part 748bp; vector 2155
Sample 2: mCherry pSB1A3 colony 2; 2H5Q; part 748bp; vector 2155


'''DNA Concentration Data'''
'''DNA Concentration Data'''
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| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
| 1. Plasmid 1 || --- || --- || ---
| 1. Plasmid 1 || 0.197 || 1.888 || 197.489
|-
|-
| 2. Plasmid 2 || --- || --- || ---
| 2. Plasmid 2 || .130 || 1.889 || 129.815
|}
|}


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| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = 746, 2155<br>2. Plasmid 2 = 746, 2155<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:http://openwetware.org/images/4/48/Screen_Shot_2015-05-14_at_1.14.53_PM.png|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA(plasmid) || 3.0 μL
| DNA(plasmid) || 3.0 μL
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'''DNA Sequencing Samples'''
 
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
 
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
 
* Submitted to DNASU on: 'date'
 
* Order Number:
 
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
# Plasmid 1 - forward primer 'name'
 
# Plasmid 1 - reverse primer 'name'
 
# Plasmid 2 - forward primer 'name'
<!-- If you need to create an entry and someone else has already used this page, add another ==Name - Date== header below their entry and click save. -->
# Plasmid 2 - reverse primer 'name'
 
|}
 
__NOTOC__

Latest revision as of 00:56, 27 September 2017

Haynes Lab Cloning Bootcamp Main project page
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Daniel Vargas - 04/29/2015

mCherry pSB1A3 using Amp / DH5α-Turbo

Inoculation 04/28/15 http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp/2015/04/28

Liquid culture 1: growth

Liquid culture 2: growth

Streak 1: growth

Streak 2: growth

Minipreps

Sample 1: mCherry pSB1A3 colony 1; 2H5Q; part 748bp; vector 2155

Sample 2: mCherry pSB1A3 colony 2; 2H5Q; part 748bp; vector 2155

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.197 1.888 197.489
2. Plasmid 2 .130 1.889 129.815


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 746, 2155
2. Plasmid 2 = 746, 2155
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.







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