Haynes Lab:Notebook/TB Biosensor/2012/06/11: Difference between revisions

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{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->
{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->
|-valign="top"
|-valign="top"
| <u>Sample</u> || <u>260/280</u> || <u>ng/µL</u>  
| <u>Sample</u> || <u>260</u> || <u>260/280</u> || <u>ng/µL</u>  
|-
|-
| crtE 1 || 1.871 || 57.761
| crtE 1 || 0.058 || 1.871 || 57.761
|-
|-
| crtE 2 || 1.961 || 63.241
| crtE 2 || 0.063 || 1.961 || 63.241
|-
|-
| crtE 3 || 1.896 || 67.32
| crtE 3 || 0.067 || 1.896 || 67.32
|-
|-
| crtB 4 || 0.786 || -1.142
| crtB 4 || -0.001 || 0.786 || -1.142
|-
|-
| crtB 5 || -1.111 || 1.053
| crtB 5 || 0.001 || -1.111 || 1.053
|-
|-
| crtB 6 || 2 || -0.413
| crtB 6 || 0 || 2 || -0.413
|-
|-
| crtI 8 || 1.913 || 103.293
| crtI 8 || 0.103 || 1.913 || 103.293
|-
|-
| crtI 9 || 1.963 || 66.049
| crtI 9 || 0.066 || 1.963 || 66.049
|-
|-
| crtI 10 || 1.892 || 54.883
| crtI 10 || 0.055  || 1.892 || 54.883
|-
|-
| crtI 11 || 1.928 || 60.177
| crtI 11 || 0.06 || 1.928 || 60.177
|-
|-
| V0120 top || 1.91 || 35.223
| V0120 top || 0.035 || 1.91 || 35.223
|-
|-
| V0120 bottom || 1.897 || 26.5
| V0120 bottom || 0.026 || 1.897 || 26.5
|}
|}


Revision as of 22:17, 11 June 2012

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Entry title

Purified frozen gel slices from 12.06.11 using Zymo Gel Purification kit. Eluted in 15ul sterile water.

Determined DNA concentrations using Take 3:

Sample 260 260/280 ng/µL
crtE 1 0.058 1.871 57.761
crtE 2 0.063 1.961 63.241
crtE 3 0.067 1.896 67.32
crtB 4 -0.001 0.786 -1.142
crtB 5 0.001 -1.111 1.053
crtB 6 0 2 -0.413
crtI 8 0.103 1.913 103.293
crtI 9 0.066 1.963 66.049
crtI 10 0.055 1.892 54.883
crtI 11 0.06 1.928 60.177
V0120 top 0.035 1.91 35.223
V0120 bottom 0.026 1.897 26.5



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