Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions
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--> | --> Wang Lab PCR machine, block A | ||
* 95°C/ 3 min. | * 95°C/ 3 min. | ||
* [95°C/ 30 sec; | * [95°C/ 30 sec; 50°C/ 30 sec; 72 °C/ 1 min.] x30 | ||
* 72 °C/ 3 min. | * 72 °C/ 3 min. | ||
* 4 °C/ ∞ | * 4 °C/ ∞ | ||
Revision as of 17:11, 8 June 2012
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Entry titlePCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift). Plasmids from 12.05.29:
PCR reaction
--> Wang Lab PCR machine, block A
> Qiagen PCR cleanup kit; elute w/ 50ul EB2O | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
