Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions
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PCR reaction | PCR reaction | ||
{| class="wikitable" border="0" cellspacing="3" <!-- PCR rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> | |||
|- | |||
| DNA (plasmid) || 1.0 | |||
|- | |||
| 10 μM primers || 1.0 | |||
|- | |||
| 2x GoTaq || 25.0 | |||
|- | |||
| dH<sub>2</sub>O || 23.0 | |||
|} | |||
Revision as of 16:48, 8 June 2012
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||
Entry titlePCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift). Plasmids from 12.05.29: Lane Part Number Gene(s) 1 K118014 RBS + crtE 2 K274100 RBS + crtE + RBS + crtI + RBS + crtB 3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 4 K118002 crtB 5 K118006 RBS + crtB 6 I742161 RBS + crtI + RBS + crtB 7 K118003 crtI 8 K118005 RBS + crtI Gene Plasmid Primer F Primer R crtE 1 crtE 2 crtE 3 crtB 2 crtB 3 crtB 4 crtB 5 crtI 2 crtI 3 crtI 7 crtI 8
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