Haynes Lab:Notebook/TB Biosensor/2012/05/24: Difference between revisions

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I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 36deg (maybe 30? check warm room temp).
I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 37deg.


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Revision as of 10:53, 25 May 2012

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The following parts are Amp resistant and from the bacteria, Pantoea ananatis. The numbers correspond to the labeling used in the lab:


1. K118014; RBS + crtE; 2012 kit plate 2 well 18H http://partsregistry.org/Part:BBa_K118014)

2. K274100; RBS + crtE + RBS + crtI + RBS + crtB; 2012 kit plate 3 well 6H http://partsregistry.org/Part:BBa_K274100

3. K274200; RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY; 2012 kit plate 3 well 6L http://partsregistry.org/Part:BBa_K274200

4. K118002; crtB; 2012 kit plate 2 well 16F http://partsregistry.org/Part:BBa_K118002

5. K118006; RBS + crtB; 2012 kit plate 2 well 16N http://partsregistry.org/Part:BBa_K118006

6. I742161; RBS + crtI + RBS + crtB; 2012 kit plate 2 well 20C http://partsregistry.org/Part:BBa_I742161

7. K118003; crtI; 2012 kit plate 2 well 16H http://partsregistry.org/Part:BBa_K118003

8. K118005; RBS + crtI; 2012 kit plate 2 well 16L http://partsregistry.org/Part:BBa_K118005


I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 37deg.


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