Haynes Lab:Notebook/Synthetic Chromatin for Cancer Research/2015/10/28: Difference between revisions
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(Autocreate 2015/10/28 Entry for Haynes_Lab:Notebook/Synthetic_Chromatin_for_Cancer_Research) |
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== | ==10-28-15== | ||
* | * Miniprep pet28 vector | ||
* Gel verification of pet28. Image in dropbox. | |||
* PCR DT017, DT028, DT040 so we can put it into the pet28 vector (they are currently in V0120). | |||
* PCR clean up for DT017, DT028, DT040, and pet28. | |||
** Protocol for PCR clean up: | |||
# Add 5x volume PB | |||
# Add 1/10 PCR volume of 3M NaAC | |||
# Bind to column (1 min @ max speed, dump) | |||
# 750ul PE (dump. 1 min @ max) | |||
# 1 min spin | |||
# Elute with 35ul of H20 | |||
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Revision as of 09:48, 14 January 2016
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10-28-15
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