Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Genes/2012/05/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(One intermediate revision by the same user not shown)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Control Genes==
*In order to get more accurate signal value readings for hotspots (not raw signal) I defined regions 250 bps to the left and right of the promoter region of each housekeeping gene. Below are the results I obtained. I can now redo GALAXY for BJ and HUVEC.  
*In order to get more accurate signal value readings for hotspots (not raw signal) I defined regions 250 bps to the left and right of the promoter region of each housekeeping gene. Below are the results I obtained. I can now redo GALAXY for BJ and HUVEC.  
*I can now do a better check on a wider variety of cell types since I have the needed check regions
*I can now do a better check on a wider variety of cell types since I have the needed check regions
*Sidenote: HUVEC comes from epithelial cells which is naturally higher in signal value.
*Sidenote: HUVEC comes from epithelial cells which is naturally higher in signal value.
[[Image:Screen_Shot_2012-05-29_at_2.19.52_AM.png]]
[[Image:Screen_Shot_2012-06-06_at_5.50.43_PM.png]]





Revision as of 11:23, 8 June 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Control Genes

  • In order to get more accurate signal value readings for hotspots (not raw signal) I defined regions 250 bps to the left and right of the promoter region of each housekeeping gene. Below are the results I obtained. I can now redo GALAXY for BJ and HUVEC.
  • I can now do a better check on a wider variety of cell types since I have the needed check regions
  • Sidenote: HUVEC comes from epithelial cells which is naturally higher in signal value.