Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/06/12

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/06/12 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==K562: cDNA Synthesis==
-
* Insert content here...
+
'''Follow cDNA Synthesis procedure from 1/10/13'''
-
 
+
<br>
 +
'''Sample Reading'''
 +
* Add 2μL of each sample plus  blank to the plate reader
 +
# K562 Sample 1: 0.4 (260), 1.143 (260/280), 340.3 (ng/mL)
 +
# K562 Sample 2: 0.4, 0.635, 280.9
 +
*Need to make 8μL solutions of the lowest concentration for each cell line
 +
# Sample 1: 6.6μL cells + 1.4 H2O = 2.2ug
 +
# Sample 2: 8μL cells = 2.5ng
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 19:48, 12 June 2013

Project name Main project page
Previous entry      Next entry

K562: cDNA Synthesis

Follow cDNA Synthesis procedure from 1/10/13
Sample Reading

  • Add 2μL of each sample plus blank to the plate reader
  1. K562 Sample 1: 0.4 (260), 1.143 (260/280), 340.3 (ng/mL)
  2. K562 Sample 2: 0.4, 0.635, 280.9
  • Need to make 8μL solutions of the lowest concentration for each cell line
  1. Sample 1: 6.6μL cells + 1.4 H2O = 2.2ug
  2. Sample 2: 8μL cells = 2.5ng
Personal tools