Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/06/12: Difference between revisions

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==Entry title==
==K562: cDNA Synthesis==
* Insert content here...
'''Follow cDNA Synthesis procedure from 1/10/13'''
 
<br>
<br>
'''Sample Reading'''
* Add 2μL of each sample plus  blank to the plate reader
# K562 Sample 1: 0.4 (260), 1.143 (260/280), 340.3 (ng/mL)
# K562 Sample 2: 0.4, 0.635, 280.9
*Need to make 8μL solutions of the lowest concentration for each cell line
# Sample 1: 6.6μL cells + 1.4 H2O = 2.2ug
# Sample 2: 8μL cells = 2.5ng


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Latest revision as of 22:45, 26 September 2017

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K562: cDNA Synthesis

Follow cDNA Synthesis procedure from 1/10/13

Sample Reading

  • Add 2μL of each sample plus blank to the plate reader
  1. K562 Sample 1: 0.4 (260), 1.143 (260/280), 340.3 (ng/mL)
  2. K562 Sample 2: 0.4, 0.635, 280.9
  • Need to make 8μL solutions of the lowest concentration for each cell line
  1. Sample 1: 6.6μL cells + 1.4 H2O = 2.2ug
  2. Sample 2: 8μL cells = 2.5ng
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