Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/06/12: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2013/06/12 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==K562: cDNA Synthesis== | ||
* | '''Follow cDNA Synthesis procedure from 1/10/13''' | ||
<br> | |||
'''Sample Reading''' | |||
* Add 2μL of each sample plus blank to the plate reader | |||
# K562 Sample 1: 0.4 (260), 1.143 (260/280), 340.3 (ng/mL) | |||
# K562 Sample 2: 0.4, 0.635, 280.9 | |||
*Need to make 8μL solutions of the lowest concentration for each cell line | |||
# Sample 1: 6.6μL cells + 1.4 H2O = 2.2ug | |||
# Sample 2: 8μL cells = 2.5ng | |||
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Revision as of 16:48, 12 June 2013
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K562: cDNA SynthesisFollow cDNA Synthesis procedure from 1/10/13
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