Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20: Difference between revisions
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* Prepared rxn setup for plates | * Prepared rxn setup for plates | ||
3x reaction: | 3x reaction: <br/> | ||
Probe master mix, (7.5 x3) 22.5 uL | Probe master mix, (7.5 x3) 22.5 uL <br/> | ||
1:10 cDNA, (2.0 x3) 6.0 uL | 1:10 cDNA, (2.0 x3) 6.0 uL <br/> | ||
dH2O, (0.2 x3) 0.6 uL | dH2O, (0.2 x3) 0.6 uL <br/> | ||
Total = (9.7 x3) 29.1 | Total = (9.7 x3) 29.1 | ||
Revision as of 11:27, 22 February 2013
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Assay Design
3x reaction: Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
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