Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20: Difference between revisions
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dH2O, (0.2 x3) 0.6 uL <br/> | dH2O, (0.2 x3) 0.6 uL <br/> | ||
Total = (9.7 x3) 29.1 | Total = (9.7 x3) 29.1 | ||
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Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | ||
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*Note: To dilute cDNA add 10uL of cDNA to 90uL of water | |||
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Revision as of 11:32, 22 February 2013
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Assay Design
3x reaction:
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