Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20

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(Autocreate 2013/02/20 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
Current revision (13:32, 22 February 2013) (view source)
(Assay Design)
 
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==Entry title==
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==Assay Design==
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* Insert content here...
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* Designed 6 plates for each cell line
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* Prepared rxn setup for plates
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3x reaction: <br/>
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Probe master mix, (7.5 x3) 22.5 uL <br/>
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1:10 cDNA, (2.0 x3) 6.0 uL <br/>
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dH2O, (0.2 x3) 0.6 uL <br/>
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Total = (9.7 x3) 29.1
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<br/>
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<br/>
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Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
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<br/>
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<br/>
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*Note: To dilute cDNA add 10uL of cDNA to 90uL of water
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Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).

  • Note: To dilute cDNA add 10uL of cDNA to 90uL of water
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