Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20

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(Assay Design)
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* Prepared rxn setup for plates
* Prepared rxn setup for plates
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3x reaction:
+
3x reaction: <br/>
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Probe master mix, (7.5 x3) 22.5 uL
+
Probe master mix, (7.5 x3) 22.5 uL <br/>
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1:10 cDNA, (2.0 x3) 6.0 uL
+
1:10 cDNA, (2.0 x3) 6.0 uL <br/>
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dH2O, (0.2 x3) 0.6 uL
+
dH2O, (0.2 x3) 0.6 uL <br/>
Total = (9.7 x3) 29.1
Total = (9.7 x3) 29.1

Revision as of 13:27, 22 February 2013

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Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).


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