Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20
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* Prepared rxn setup for plates | * Prepared rxn setup for plates | ||
| - | 3x reaction: | + | 3x reaction: <br/> |
| - | Probe master mix, (7.5 x3) 22.5 uL | + | Probe master mix, (7.5 x3) 22.5 uL <br/> |
| - | 1:10 cDNA, (2.0 x3) 6.0 uL | + | 1:10 cDNA, (2.0 x3) 6.0 uL <br/> |
| - | dH2O, (0.2 x3) 0.6 uL | + | dH2O, (0.2 x3) 0.6 uL <br/> |
Total = (9.7 x3) 29.1 | Total = (9.7 x3) 29.1 | ||
| - | + | <br/> | |
| + | <br/> | ||
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | ||
| - | + | <br/> | |
| - | + | <br/> | |
| + | *Note: To dilute cDNA add 10uL of cDNA to 90uL of water | ||
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Assay Design
3x reaction:
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