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Project name
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Cells in Trizole
K562
- Expand cells to 10cm petri dishes for 10mL of cells
- For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total)
- Spin tubes at room temperature @ 3000 RPM for 5 min
- Dispose of supernatant
- Add 500μL of trizol to each pellet
- Store @ -80°C
SK-N-SH
- From the 6 well plate aspirate off the growth media
- Add 500μL of trizol directly to the 6 well plate
- Let sit for 5 min @ room temperature under the hood
- Use 100m0mL pipet to scrape and pipet up and down to break up cells
- Transfer 500μL into each tube
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