Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/18

From OpenWetWare

< Haynes Lab:Notebook | Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2 | 2012 | 12
Revision as of 14:38, 11 January 2013 by Caroline Hom (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Cells in Trizole

  • Cells in trizole at 80°C

K562

  1. Expand cells to 10cm petri dishes for 10mL of cells
  2. For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total)
  3. Spin tubes at room temperature @ 3000 RPM for 5 min
  4. Dispose of supernatant
  5. Add 500μL of trizol to each pellet
  6. Store @ -80°C

SK-N-SH

  1. From the 6 well plate aspirate off the growth media
  2. Add 500μL of trizol directly to the 6 well plate
  3. Let sit for 5 min @ room temperature under the hood
  4. Use 100m0mL pipet to scrape and pipet up and down to break up cells
  5. Transfer 500μL into each tube


Personal tools