Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/18: Difference between revisions

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Latest revision as of 22:21, 26 September 2017

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Cells in Trizole

  • Cells in trizole at 80°C

K562

  1. Expand cells to 10cm petri dishes for 10mL of cells
  2. For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total)
  3. Spin tubes at room temperature @ 3000 RPM for 5 min
  4. Dispose of supernatant
  5. Add 500μL of trizol to each pellet
  6. Store @ -80°C

SK-N-SH

  1. From the 6 well plate aspirate off the growth media
  2. Add 500μL of trizol directly to the 6 well plate
  3. Let sit for 5 min @ room temperature under the hood
  4. Use 100m0mL pipet to scrape and pipet up and down to break up cells
  5. Transfer 500μL into each tube


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