Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14

From OpenWetWare

Jump to: navigation, search
(→Splitting Cells)
Current revision (12:35, 16 November 2012) (view source)
(→Splitting Cells)
 
Line 18: Line 18:
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
-
 
-
 
-
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells
1. 25mL diluted cells

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"

Retrieved from "http://openwetware.org/wiki/Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2/2012/11/14"
Personal tools