Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14

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(Splitting Cells)
(Splitting Cells)
Line 12: Line 12:
5X diluted cells
5X diluted cells
-
1. 25mL diluted cells <br>
+
1. 25mL diluted cells  
 +
</br>
*5mL cells + 20mL medium in a 50mL conical vial
*5mL cells + 20mL medium in a 50mL conical vial
*Pipette up and down
*Pipette up and down

Revision as of 11:34, 16 November 2012

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Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells 1. 25mL diluted cells </br>

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"



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