Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Caroline Hom (talk | contribs) |
Caroline Hom (talk | contribs) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Splitting Cells== | ==Splitting Cells== | ||
Seed Culture | '''Seed Culture''' | ||
# 12-well plate | # 12-well plate | ||
# 75mL flask | # 75mL flask | ||
5X diluted cells | '''5X diluted cells''' | ||
<br/> | |||
1. 25mL diluted cells | 1. 25mL diluted cells | ||
*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down | ||
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 09:35, 16 November 2012
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Splitting CellsSeed Culture
5X diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | |
