Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions
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==Splitting Cells== | ==Splitting Cells== | ||
Seed Culture | '''Seed Culture''' | ||
# 12-well plate | # 12-well plate | ||
# 75mL flask | # 75mL flask | ||
5X diluted cells | '''5X diluted cells''' | ||
<br/> | |||
1. 25mL diluted cells | 1. 25mL diluted cells | ||
*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down | ||
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
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Revision as of 09:35, 16 November 2012
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Splitting CellsSeed Culture
5X diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" |