Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14

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(Splitting Cells)
Line 12: Line 12:
5X diluted cells
5X diluted cells
-
#25mL diluted cells
+
1. 25mL diluted cells
*5mL cells + 20mL medium in a 50mL conical vial
*5mL cells + 20mL medium in a 50mL conical vial
*Pipette up and down
*Pipette up and down
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
-
# 8mL medium + 2mL cells in 75mL flask labeled "PS4"
+
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"

Revision as of 12:33, 16 November 2012

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Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells 1. 25mL diluted cells

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"



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