Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14

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(Splitting Cells)
Current revision (12:35, 16 November 2012) (view source)
(Splitting Cells)
 
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==Splitting Cells==
==Splitting Cells==
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Seed Culture
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'''Seed Culture'''
# 12-well plate
# 12-well plate
# 75mL flask
# 75mL flask
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5X diluted cells
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'''5X diluted cells'''
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<br/>
1. 25mL diluted cells  
1. 25mL diluted cells  
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<br/>
 
*5mL cells + 20mL medium in a 50mL conical vial
*5mL cells + 20mL medium in a 50mL conical vial
*Pipette up and down
*Pipette up and down
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
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Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells
1. 25mL diluted cells

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"

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