Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14
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< Haynes Lab:Notebook | Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2 | 2012 | 11(Difference between revisions)
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Current revision (12:35, 16 November 2012) (view source) (→Splitting Cells) |
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==Splitting Cells== | ==Splitting Cells== | ||
| - | Seed Culture | + | '''Seed Culture''' |
# 12-well plate | # 12-well plate | ||
# 75mL flask | # 75mL flask | ||
| - | 5X diluted cells | + | '''5X diluted cells''' |
| - | 1. 25mL diluted cells | + | <br/> |
| + | 1. 25mL diluted cells | ||
*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down | ||
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
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Splitting CellsSeed Culture
5X diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | |
