Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/09/17: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2012/09/17 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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==Entry title== | ==Entry title== | ||
The previous attempt to get the cells started officially did not work. The cells did not attach and were floating, even after three days of incubation at 37C. I did another thaw and this time I did the following: | |||
#Added thawed cells to 5 mL pure FBS in 15 mL conical tubes | |||
#Spun at 1000 rpm for 4 minutes | |||
#Aspirated off the FBS (to get rid of any toxins from the freezing solution) | |||
#Resuspended the cell pellet in 1mL 20% FBS/ 1% pen-strep DMEM media | |||
#Added the resuspended cells to 3 mL of 20% FBS/ 1% pen-strep DMEM media in a 6-well culture dish (one cell line per well). This gives the cells less surface area to stick to and crowds them together, which sometimes promotes cell growth. | |||
The cell lines I thawed are | |||
#HepG2 | |||
#Luc #4 | |||
#23;4;9 Luc EED | |||
Revision as of 13:43, 31 October 2012
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Entry titleThe previous attempt to get the cells started officially did not work. The cells did not attach and were floating, even after three days of incubation at 37C. I did another thaw and this time I did the following:
The cell lines I thawed are
|