Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29

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(Autocreate 2012/08/29 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
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==Entry title==
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==Digestion==
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* Insert content here...
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* We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
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* Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126
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<br>
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u>
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| rowspan="7" | <u>Expected:</u><br>1,2. KAH123 = 8002<br>3,4. KAH124 = 8174<br>5,6. KAH125 = 8246<br>
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| rowspan="7" | [[Image:KAH_121509_gel1.tif|300px|E/P digests 12/15/09]]<br>15 μL/lane; 1% agarose -->
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|-
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| DNA(plasmid) || 3.0 μL || -
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|-
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| 10X buffer || 1.5 || 4.5
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|-
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| EcoRI || 1.0 || 3.0
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|-
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| PstI || 1.0 || 3.0
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|-
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| dH<sub>2</sub>O || 8.5 || 25.5
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|-
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| &nbsp; || 15 μL --> 37°C/ ~15 min.
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|}

Revision as of 15:28, 5 September 2012

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Digestion

  • We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
  • Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126


Reagent Volume Expected:
1,2. KAH123 = 8002
3,4. KAH124 = 8174
5,6. KAH125 = 8246
E/P digests 12/15/09
15 μL/lane; 1% agarose -->
DNA(plasmid) 3.0 μL -
10X buffer 1.5 4.5
EcoRI 1.0 3.0
PstI 1.0 3.0
dH2O 8.5 25.5
  15 μL --> 37°C/ ~15 min.


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