Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Caroline Hom (talk | contribs) (Autocreate 2012/08/22 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
Caroline Hom (talk | contribs) |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==QiaPrep Spin== | ||
# Transfer 2mL of culture to round small round bottom tubes | |||
# Divide plate and label (this will be your back up culture) | |||
# Drag tip from tube lightly across the agar. Put the tip back in the tube. (Use forceps) | |||
# Centrifuge for 3 min (make sure to balance and keep centrifuge at top speed) | |||
# Dump waste from tubes | |||
# Resuspend pellet in 250uL of PI buffer then vortex | |||
# Add 250uL of P2 buffer and invert 6x | |||
# Add 350uL of N3 buffer and invert 10x | |||
# Centrifuge for 10 min | |||
# Label column collection tubes (blue) and dump you centrifuged liquid in columns | |||
# Spin for 1 min and dump waste | |||
# Add 750uL of PE buffer | |||
# Centrifuge for 1 min, dump, centrifuge for 1 min | |||
# Place liquid in 1.5mL microcentrifuge tube and add 75uL of EB buffer directly above the white filter | |||
# Centrifuge for 2 min toss blue column | |||
Revision as of 12:11, 5 September 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
QiaPrep Spin
|