Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/21: Difference between revisions

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==Entry title==
==Entry title==
(1) Growth
(1) Growth
<br>
<br>
<br>
(2) No Growth (control)
(2) No Growth (control)
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#2. Mix
#2. Mix
<br>
<br>
#1. Label round bottom tubes
# Label round bottom tubes
#2. With a serological pipet add 2mL of Amp LB per tube
# With a serological pipet add 2mL of Amp LB per tube
#3. Use yellow pipets to culture, toss in tubes
# Use yellow pipets to culture, toss in tubes
#4. Rewrap the plate, store in fridge
# Rewrap the plate, store in fridge
#5. Leave caps on tubes, store in incubator
# Leave caps on tubes, store in incubator
#6. Turn shaker on, make sure H2O is in tupperware
# Turn shaker on, make sure H2O is in tupperware





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Entry title

(1) Growth

(2) No Growth (control)
For Amp LB

  1. 1. Dilute x1000 (100ug/Ml)

Ex: 400uL of amp/400mL of LB Broth)

  1. 2. Mix


  1. Label round bottom tubes
  2. With a serological pipet add 2mL of Amp LB per tube
  3. Use yellow pipets to culture, toss in tubes
  4. Rewrap the plate, store in fridge
  5. Leave caps on tubes, store in incubator
  6. Turn shaker on, make sure H2O is in tupperware