Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/20: Difference between revisions
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Using the KAH126 insert with the biobrick BBa_J176062 | Using the KAH126 insert with the biobrick BBa_J176062 | ||
Vector MV2 (mammalian) with birobrick BBa_J176122 | Vector MV2 (mammalian) with birobrick BBa_J176122 | ||
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DNA Prep | DNA Prep | ||
# 2 sample tubes (0.5mL) | |||
# Add 10uL H20 and 0.5uL of DNA to (1) and 10uL H20 to (2) | |||
# Need 30uL of cell per transformation (there is 200uL total in a stock tube) * Cell Line (DH5 | |||
* Cell Line (DH5 |
Revision as of 11:58, 5 September 2012
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Entry titleUsing the KAH126 insert with the biobrick BBa_J176062
Vector MV2 (mammalian) with birobrick BBa_J176122
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