Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2

From OpenWetWare

< Haynes Lab:Notebook(Difference between revisions)
Jump to: navigation, search
(New page: {|{{table}} width="800" |- |style="background-color: #800000;" align="center"| <!-- ## START calendar column --> <LNCalendar></LNCalendar> <!-- ## END calendar column --> |align="center" s...)
Current revision (20:55, 16 April 2013) (view source)
(Notes)
 
(6 intermediate revisions not shown.)
Line 13: Line 13:
|-  
|-  
|colspan="2"|
|colspan="2"|
-
==Project Description/Abstract==
+
==Phase 3: Expression==
-
* Since a comprehensive ChIP map is not available for osteosarcoma, I will identify genes associated with H3K27me3 in liver (HepG2) and fibroblast (BJ) cell lines. ChIP is available through the Encyclopedia of DNA Elements (ENCODE) (3). I will extract a list of genes with H3K27me3 near the transcription start site (promoter) as well as genes with a broad distribution of H3K27me3 to determine later on if H3K27me3 localization influences Pc-TF function. I will also determine which CBX isoforms are at each locus to determine variations between H3K27me3 sites.
+
At this stage the necessary bioinformatics has been performed in order to identify which genes could be potentially turned on by PcTF. Using Real-Time qRT-PCR we can determine which genes form that list are up-regulated and what further analysis need to be performed. We will also continue research by seeing how cell phenotype and growth are affected by PcTF.
==Notes==
==Notes==
-
* Phase 1 in progress
+
* Phase 3 is in progress

Current revision

Search this Project

Customize your entry pages

Phase 3: Expression

At this stage the necessary bioinformatics has been performed in order to identify which genes could be potentially turned on by PcTF. Using Real-Time qRT-PCR we can determine which genes form that list are up-regulated and what further analysis need to be performed. We will also continue research by seeing how cell phenotype and growth are affected by PcTF.

Notes

  • Phase 3 is in progress


Recent changes



Personal tools