Haynes Lab:Notebook/SynBERC Scholars 2013/2014/03/18: Difference between revisions

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==Entry title==
==03/18/14==
* Insert content here...
* Dilution-limited PCR: Kozak plasmid and Luc14 genomic DNA
 
 
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'''Dilution-limited PCR'''
* K = Kozak plasmid DNA, initial concentration = 243 ng/μL
* L = Luc 14 genomic DNA, initial concentration = 217 ng/μL
 
Dilutions for PCR Reactions
* K1 = 10<sup>-3</sup>
* K2 = 10<sup>-6</sup>
* K3 = 10<sup>-9</sup>
* K4 = 10<sup>-12</sup>





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03/18/14

  • Dilution-limited PCR: Kozak plasmid and Luc14 genomic DNA



Dilution-limited PCR

  • K = Kozak plasmid DNA, initial concentration = 243 ng/μL
  • L = Luc 14 genomic DNA, initial concentration = 217 ng/μL

Dilutions for PCR Reactions

  • K1 = 10-3
  • K2 = 10-6
  • K3 = 10-9
  • K4 = 10-12