Haynes Lab:Notebook/SynBERC Scholars 2013/2013/12/17: Difference between revisions
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# Did this three times for sample #1 (all components) and sample #2 (forward primer + template DNA). ''Note: Will continue with other samples after winter break'' | # Did this three times for sample #1 (all components) and sample #2 (forward primer + template DNA). ''Note: Will continue with other samples after winter break'' | ||
# Zazu used the video function on his iPhone 5: set camera in cradle, started recording, shielded away all light with lid, removed lid, stopped recording. Saved a frame from the movie where light was blocked out as the official image. | # Zazu used the video function on his iPhone 5: set camera in cradle, started recording, shielded away all light with lid, removed lid, stopped recording. Saved a frame from the movie where light was blocked out as the official image. | ||
* Image J | |||
# Drag & drop image into Image J | |||
* Cropped image with square selection tool | |||
* Selected area with circle selection tool | |||
* Reproduced exact same circle selection in next image by using Edit > Selection > Restore selection | |||
* Under Analyze > Set Measurements make sure the integrated density option is selected. Click okay. | |||
* Activate the window of the image you want to measure, hit command-M (or select Analyze > Measure). Window will appear with numbers. Record integrated density in a table or spreadsheet. | |||
* Repeat this step for next image | |||
# IMG_1989.png = 375909 | |||
# IMG_1981-1.png = 397251 | |||
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Revision as of 15:37, 17 December 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||
12/17/13
Part 1: PCR
N.F. H2O = nuclease-free water from Promega kit
Part 2: Fluorimeter
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