Haynes Lab:Notebook/SynBERC Scholars 2013/2013/12/17: Difference between revisions

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Part 1: PCR
Part 1: PCR
* Use the Open PCR machine to amplify segment from "CMV" plasmid DNA
* Use the Open PCR machine to amplify segment from "CMV" plasmid DNA
{|
| align="center" style="background:#f0f0f0;"|'''Reagent'''
| align="center" style="background:#f0f0f0;"|'''1'''
| align="center" style="background:#f0f0f0;"|'''2'''
| align="center" style="background:#f0f0f0;"|'''3'''
| align="center" style="background:#f0f0f0;"|'''4'''
| align="center" style="background:#f0f0f0;"|'''5'''
|-
| 2x PCR mix||50||50||50||50||50
|-
| P0001||1||1||0||0||1
|-
| P0002||1||0||1||0||1
|-
| DNA template||0.5||0.5||0.5||0.5||0
|-
| N.F. H2O||47.5||48.5||48.5||49.5||48
|-
| Final vol||100||100||100||100||100
|}




Revision as of 14:44, 17 December 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/17/13

  • First trail run of OpenPCR & fluorimeter


Part 1: PCR

  • Use the Open PCR machine to amplify segment from "CMV" plasmid DNA
Reagent 1 2 3 4 5
2x PCR mix 50 50 50 50 50
P0001 1 1 0 0 1
P0002 1 0 1 0 1
DNA template 0.5 0.5 0.5 0.5 0
N.F. H2O 47.5 48.5 48.5 49.5 48
Final vol 100 100 100 100 100



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