Haynes Lab:Notebook/Short Projects/2015/05/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Ben Nyer - 29/05/15== | ||
'''Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene''' | '''Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene''' | ||
Latest revision as of 01:00, 27 September 2017
 Haynes Lab - Short Projects
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Ben Nyer - 29/05/15Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene Transformation results:
Transformation efficiency is pretty low. If plasmid prep/verification doesn't work, try longer transformation protocol using remaining ligation product.
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