Haynes Lab:Notebook/Short Projects/2015/05/28: Difference between revisions
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Bio-Rad C1000 Touch Thermocycler: Olig PNK-Ann | |||
* 37°C, 30 min | * 37°C, 30 min | ||
* 95°C, 5 min | * 95°C, 5 min | ||
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Bio-Rad C1000 Touch Thermocycler: BbsI DigLig | |||
* 6x [37°C, 5 min; 23°C, 5 min] | * 6x [37°C, 5 min; 23°C, 5 min] | ||
* 4°C, ∞ | * 4°C, ∞ |
Revision as of 15:54, 28 May 2015
Haynes Lab - Short Projects | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||
Ben Nyer - 28/05/2015Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene
Phosphorylate and anneal oligo pairs
Bio-Rad C1000 Touch Thermocycler: BbsI DigLig
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