Haynes Lab:Notebook/Short Projects/2014/10/17

From OpenWetWare
Jump to navigationJump to search
Haynes Lab - Short Projects <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

10/17/14 - Jonah Thomas

  • Performed transfections for KAH60/pcVn plasmids



HEK293 Transfection w/ KAH60/pcVn

  • The Haynes Lab protocol for transfections was followed

Note: The plasmid concentration used for this transfection was ~58ng/μL meaning the total plasmid amount used to transfect each well was ~1160ng

  • A total of 2 wells were transfected with the KAH60/pcVn plasmids
    • Two mock transfections were also completed as a control for each plate
  • In order to improve plasmid uptake during the transfection, one plate (Plate 2) was spun in a centrifuge and Plate 1 was left to incubate as a control

The results of this transfection with be observed and reported on Monday, October 20, 2014.



Plan of Action: Week of 10/20/14

  • Monday (10/20): Record and report the results of the transfection mini-experiment. Also, perform transduction with E. coli for new plasmid mini preps
  • Tuesday (9/23): Check bacterial colonies and incubate cell colonies in LB broth for new mini preps
  • Wednesday (9/24): Perform 4 minipreps
  • Thursday (9/25): Perform new set of transfections
  • Friday (9/26): Check status of transfections



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Short_Projects/2014/10/17&oldid=830440"