Haynes Lab:Notebook/Short Projects/2014/09/23: Difference between revisions
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Jonah Thomas (talk | contribs) (Autocreate 2014/09/23 Entry for Haynes_Lab:Notebook/Short_Projects) |
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== | ==09/23/14 - Jonah Thomas== | ||
* | * Prepared pen-strep free DMEM | ||
* Performed transfection of HEK293 Gal4-EED -dox and +dox cells | |||
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'''Transfection''' | |||
* Transfecting 6 wells total with KAH60/vnPc from Vial #2 | |||
** 3 -dox wells | |||
** 3 +dox wells | |||
** All other wells will be mock transfected | |||
9.1μL of plasmid will be brought up to 20μL using water | |||
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Revision as of 14:12, 23 September 2014
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09/23/14 - Jonah Thomas
Transfection
9.1μL of plasmid will be brought up to 20μL using water |