Haynes Lab:Notebook/Short Projects/2014/09/19

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09/19/14 - Jonah Thomas

  • Failed to remove plates containing transformed bacterial cells from incubator and thus, the cells became overgrown
  • Obtained 6 vials of plasmid DNA
  • Measured the concentrations of the available plasmid DNA
  • Performed a 1:4 passage of the dox- and dox+ cells in a T-75 flask

Plasmid Concentration Measurement

Note: Measurements taken using Biotek machine - Take 3 plate

  • Thawed 6 vials of plasmid DNA at room temperature
    • Labeled each vial 1-6, respectively
  • Placed 2μL of plasmid on the Take 3 Plate according to Table 1 below:
Take 3 Plate Well Plasmid OD260 260/280 Concentration [ng/μL]
A1 Blank N/A N/A N/A
B1 KAH60/vnPc 0.07 1.82 70.44
C1 KAH60/vnPc 0.22 1.75 219.73
D1 KAH59/MV5 0.24 1.85 241.06
E1 KAH54/vnPc 0.36 1.79 362.24
F1 KAH54/vnPc 0.26 1.81 257.63
G1 KAH59/vnPc 0.21 1.78 213.99


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