Haynes Lab:Notebook/Short Projects/2014/09/19: Difference between revisions
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'''Plan of Action: Week of 9/22/14''' | '''Plan of Action: Week of 9/22/14''' | ||
* <u>Monday (9/22)<u>: Transfer -dox and +dox cells to 6-well plates | * <u>Monday (9/22)</u>: Transfer -dox and +dox cells to 6-well plates | ||
* <u>Tuesday (9/23)<u>: Check cell growth | * <u>Tuesday (9/23)</u>: Check cell growth | ||
* <u>Wednesday (9/24)<u>: Perform cell transfections with KAH60/vnPc (Vial 2) | * <u>Wednesday (9/24)</u>: Perform cell transfections with KAH60/vnPc (Vial 2) | ||
** Be sure to all do mock transfections! | ** Be sure to all do mock transfections! | ||
** Depending on cell growth, this may be able to be completed on Tuesday | ** Depending on cell growth, this may be able to be completed on Tuesday | ||
* <u>Thursday (9/25)<u>: Check cell growth | * <u>Thursday (9/25)</u>: Check cell growth | ||
* <u>Friday (9/26)<u>: Check cell growth | * <u>Friday (9/26)</u>: Check cell growth | ||
* On either Thurs. or Friday, the fluorescent microscope will be used to confirm a successful transfection of cells | * On either Thurs. or Friday, the fluorescent microscope will be used to confirm a successful transfection of cells |
Revision as of 15:34, 19 September 2014
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09/19/14 - Jonah Thomas
Note: Measurements taken using Biotek machine - Take 3 plate
Note: It was calculated that 9.102μL of the KAH60/vnPc plasmid (Vial 2) will be needed to reach the 2000ng mass necessary for plasmid for cell transfection. Additionally, there appeared to be ~45-50μL of this plasmid available
Note: Upon confirming a successful transfection, a cell count will be completed as well as a luciferase assay
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