Haynes Lab:Notebook/Short Projects/2014/09/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 24: Line 24:
{| {{table}}
{| {{table}}
|-
|-
| Take 3 Plate Well || Plasmid || OD260 || 260/280 || Concentration [ng/μL]
| Take 3 Plate Well/Vial Number || Plasmid || OD260 || 260/280 || Concentration [ng/μL]
|-
|-
| A1 || Blank || N/A || N/A || N/A
| A1 || Blank || N/A || N/A || N/A
|-
|-
| B1 || KAH60/vnPc || 0.07 || 1.82 || 70.44
| B1/1 || KAH60/vnPc || 0.07 || 1.82 || 70.44
|-
|-
| C1 || KAH60/vnPc || 0.22 || 1.75 || 219.73
| '''C1/2''' || '''KAH60/vnPc''' || '''0.22''' || '''1.75''' || '''219.73'''
|-
|-
| D1 || KAH59/MV5 || 0.24 || 1.85 || 241.06
| D1/3 || KAH59/MV5 || 0.24 || 1.85 || 241.06
|-
|-
| E1 || KAH54/vnPc || 0.36 || 1.79 || 362.24
| E1/4 || KAH54/vnPc || 0.36 || 1.79 || 362.24
|-
|-
| F1 || KAH54/vnPc || 0.26 || 1.81 || 257.63
| F1/5 || KAH54/vnPc || 0.26 || 1.81 || 257.63
|-
|-
| G1 || KAH59/vnPc || 0.21 || 1.78 || 213.99
| G1/6 || KAH59/vnPc || 0.21 || 1.78 || 213.99
|}
|}


Revision as of 13:15, 19 September 2014

Haynes Lab - Short Projects <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

09/19/14 - Jonah Thomas

  • Failed to remove plates containing transformed bacterial cells from incubator and thus, the cells became overgrown
  • Obtained 6 vials of plasmid DNA
  • Measured the concentrations of the available plasmid DNA
  • Performed a 1:4 passage of the dox- and dox+ cells in a T-75 flask

Plasmid Concentration Measurement

Note: Measurements taken using Biotek machine - Take 3 plate

  • Thawed 6 vials of plasmid DNA at room temperature
    • Labeled each vial 1-6, respectively
  • Placed 2μL of plasmid on the Take 3 Plate according to Table 1 below:
Take 3 Plate Well/Vial Number Plasmid OD260 260/280 Concentration [ng/μL]
A1 Blank N/A N/A N/A
B1/1 KAH60/vnPc 0.07 1.82 70.44
C1/2 KAH60/vnPc 0.22 1.75 219.73
D1/3 KAH59/MV5 0.24 1.85 241.06
E1/4 KAH54/vnPc 0.36 1.79 362.24
F1/5 KAH54/vnPc 0.26 1.81 257.63
G1/6 KAH59/vnPc 0.21 1.78 213.99


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Short_Projects/2014/09/19&oldid=822164"