Haynes Lab:Notebook/Short Projects/2014/09/18: Difference between revisions

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* Use 0.2 mL PCR strip tubes
* Be sure to leabel each one
* Place in a plastic baggie (recycled)
* Use tape to secure baggie with tubes to order form




Revision as of 11:44, 19 September 2014

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09/18/14 - Meli'sa Crawford

  • Plasmid sequencing - planning


Plasmid sequencing - planning

Tube Primer (1 μL of 10 μM) DNA (200 ng) [DNA] Vol DNA Vol 10 μM primer Vol dH2O
1. P0001 BD003 128 ng/μL 1.6 μL 1.0 μL 7.4 μL
2. P0002 BD003 128 ng/μL 1.6 μL 1.0 μL 7.4 μL
3. P0001 BD004 141 ng/μL 1.4 μL 1.0 μL 7.6 μL
4. P0002 BD004 141 ng/μL 1.4 μL 1.0 μL 7.6 μL
5. P0001 BD005 290.2 ng/μL 0.7 μL 1.0 μL 8.3 μL
6. P0002 BD005 290.2 ng/μL 0.7 μL 1.0 μL 8.3 μL
7. P0001 BD006 255.3 ng/μL 0.8 μL 1.0 μL 8.2 μL
8. P0002 BD006 255.3 ng/μL 0.8 μL 1.0 μL 8.2 μL
  • Use 0.2 mL PCR strip tubes
  • Be sure to leabel each one
  • Place in a plastic baggie (recycled)
  • Use tape to secure baggie with tubes to order form




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