Haynes Lab:Notebook/Short Projects/2014/09/16: Difference between revisions
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== | ==09/16/14 - Jonah Thomas== | ||
* | * Transformation - KAH60/pcVN into DH5α-turbo | ||
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'''Transformation''' | |||
* HEK cells did not express mCherry after transfection | |||
** Discarded all samples | |||
* Therefore, we will try to obtain higher quality plasmid by transforming the stock into bacteria | |||
* After transformation & growth, we will do a miniprep to get fresh plasmid DNA | |||
** To check quality of DNA, we will do a restriction digest | |||
Revision as of 15:20, 16 September 2014
 Haynes Lab - Short Projects
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09/16/14 - Jonah Thomas
Transformation
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