Haynes Lab:Notebook/Short Projects/2014/09/16: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 18: Line 18:
* After transformation & growth, we will do a miniprep to get fresh plasmid DNA
* After transformation & growth, we will do a miniprep to get fresh plasmid DNA
** To check quality of DNA, we will do a restriction digest
** To check quality of DNA, we will do a restriction digest
Transformation samples
# KAH60/VNpc (from Harvard stocks) - 0.5 μL in 10 μL dH<sub>2</sub>O
# 10 μL dH<sub>2</sub>O
* Will check for colonies tomorrow morning




Revision as of 15:21, 16 September 2014

Haynes Lab - Short Projects <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

09/16/14 - Jonah Thomas

  • Transformation - KAH60/pcVN into DH5α-turbo


Transformation

  • HEK cells did not express mCherry after transfection
    • Discarded all samples
  • Therefore, we will try to obtain higher quality plasmid by transforming the stock into bacteria
  • After transformation & growth, we will do a miniprep to get fresh plasmid DNA
    • To check quality of DNA, we will do a restriction digest

Transformation samples

  1. KAH60/VNpc (from Harvard stocks) - 0.5 μL in 10 μL dH2O
  2. 10 μL dH2O
  • Will check for colonies tomorrow morning



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Short_Projects/2014/09/16&oldid=820877"