Haynes Lab:Notebook/Short Projects/2014/09/10: Difference between revisions
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# In a 50 mL conical, add '''25.7 growth medium''' (which is 28 - 2.3) | # In a 50 mL conical, add '''25.7 growth medium''' (which is 28 - 2.3) | ||
# Add '''2.3 mL of harvested cells''' to the 50 mL conical. | # Add '''2.3 mL of harvested cells''' to the 50 mL conical. | ||
# Get a 10 mL pipette and mix the cells by | # Get a 10 mL pipette and mix the cells by pipetting up and down (>3 times). | ||
# Draw up and dispense 4 mL of the suspended cells into each well in the 6-well plate. | # Draw up and dispense 4 mL of the suspended cells into each well in the 6-well plate. | ||
Revision as of 11:29, 22 September 2014
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09/10/14 - Jonah Thomas
Cell culture
Note: Do this whole procedure separately for the dox- and the dox+ cells
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