Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/09/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">U-2 OS Transfection, Day 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Overview== | ==Overview== | ||
* Photographing U-2 OS plate (day 1 & day 2 samples) using phase & mCherry | |||
* Harvesting U-2 OS day 1 samples (2 biological replicates, 1 negative control) | |||
** flow cytometry of day 1 samples | |||
** TRIzol digestion of cells and storage at -80 °C | |||
==Fluorescence Microscopy== | |||
Well 1<br> | |||
[[Image:2015-09-02_Well_1_KAH126-MV2_phase_mCherry_10x.jpg | 500px]] | |||
Well 2<br> | |||
[[Image:2015-09-02_Well_2_KAH126-MV2_phase_mCherry_10x.jpg | 500px]] | |||
Well 3<br> | |||
[[Image:2015-09-02_Well_3_no_plasmid_phase_10x.jpg | 500px]] | |||
Well 4<br> | |||
[[Image:2015-09-02_Well_4_KAH126-MV2_phase_mCherry_10x.jpg | 500px]] | |||
Well 5<br> | |||
[[Image:2015-09-02_Well_5_KAH126-MV2_phase_mCherry_10x.jpg | 500px]] | |||
Well 6<br> | |||
[[Image:2015-09-02_Well_6_no_plasmid_phase_10x.jpg | 500px]] | |||
==Flow Cytometry== | |||
Well 1 (KAH126-MV2)<br> | |||
[[Image:2015-09-02_Well_1_FC.png | 500px]] | |||
Well 2 (KAH126-MV2)<br> | |||
[[Image:2015-09-02_Well_2_FC.png | 500px]] | |||
Well 3 (no plasmid)<br> | |||
[[Image:2015-09-02_Well_3_FC.png | 500px]] | |||
==Results== | |||
Transfection efficiency as measured by flow cytometry is ~40% for replicate A in Well 1, and ~60% for replicate B in Well 2. Total cell count is approximately 400,00 cells for Wells 1 and 2 and approximately 800,000 cells in Well 3. | |||
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Latest revision as of 01:06, 27 September 2017
 U-2 OS Transfection, Day 1
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Overview
Fluorescence MicroscopyWell 1 Well 2 Well 3 Well 4 Well 5 Well 6
Flow CytometryWell 1 (KAH126-MV2)
ResultsTransfection efficiency as measured by flow cytometry is ~40% for replicate A in Well 1, and ~60% for replicate B in Well 2. Total cell count is approximately 400,00 cells for Wells 1 and 2 and approximately 800,000 cells in Well 3.
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