Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Neon transfection of K562 cell line</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Neon transfection of K562 cell line</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Treat samples in duplicate, for 12 samples in total. | Treat samples in duplicate, for 12 samples in total. | ||
==Notes== | |||
Culture density: 240,000 cells/mL * 5 mL culture = 1.2E6 cells total | |||
To make 1E7 cells/mL concentration, spin down and resuspend in 120 µL Resuspension Buffer R. | |||
pMaxGFP concentration: 2000 ng/µL | |||
KAH126-MV2 concentration: 809 ng/µL | |||
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Latest revision as of 00:59, 27 September 2017
Neon transfection of K562 cell line | Main project page Previous entry Next entry |
OverviewTrying transfection of K562 again, this time using the Neon transfection system. Protocol
Samples:
Treat samples in duplicate, for 12 samples in total. NotesCulture density: 240,000 cells/mL * 5 mL culture = 1.2E6 cells total To make 1E7 cells/mL concentration, spin down and resuspend in 120 µL Resuspension Buffer R. pMaxGFP concentration: 2000 ng/µL KAH126-MV2 concentration: 809 ng/µL
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