Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transfection optimization of K562</span> | ||
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==Overview== | ==Overview== | ||
Measured cell density of K562 culture in antibiotic-free medium at 490,000 cells/mL. At 9 mL total culture, that's 4,410,000 cells total. Ideal cell density for transfection with lipofectamine in 24-well plate is 0.5-2E5 cells per well. Assume 1 extra mL of culture as buffer. | |||
4.90E5 cells/mL * x mL = 0.5E5 cells/mL * 25 mL | |||
x = 12.5E5 cells / (4.9E5 cells/mL) | |||
x = 2.55 mL | |||
25 - 2.55 = 22.45 mL medium | |||
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Revision as of 09:48, 22 May 2015
 Transfection optimization of K562
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OverviewMeasured cell density of K562 culture in antibiotic-free medium at 490,000 cells/mL. At 9 mL total culture, that's 4,410,000 cells total. Ideal cell density for transfection with lipofectamine in 24-well plate is 0.5-2E5 cells per well. Assume 1 extra mL of culture as buffer. 4.90E5 cells/mL * x mL = 0.5E5 cells/mL * 25 mL x = 12.5E5 cells / (4.9E5 cells/mL) x = 2.55 mL 25 - 2.55 = 22.45 mL medium
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