Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DNA Concentration of pMaxGFP</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DNA Concentration of pMaxGFP</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 23: | Line 23: | ||
| pMaxGFP pre-concentration||486.333||7.966264997||1.888 | | pMaxGFP pre-concentration||486.333||7.966264997||1.888 | ||
|- | |- | ||
| evap conc.||2090. | | evap conc.||2090.9||17.05||1.90 | ||
|- | |- | ||
| evap conc, 10x dil||190. | | evap conc, 10x dil||190.1||1.86||1.91 | ||
|- | |- | ||
| precip conc.||2297. | | precip conc.||2297.7||43.69||1.90 | ||
|- | |- | ||
| precip conc, 10x dil||205. | | precip conc, 10x dil||205.7||5.06||1.82 | ||
|} | |} | ||
Latest revision as of 00:58, 27 September 2017
DNA Concentration of pMaxGFP | Main project page Previous entry Next entry | ||||||||||||||||||||||||
OverviewThe sample left to dry overnight has evaporated down to a volume of under 30 µL. I'm taking another 500 µL of the plasmid and performing a sodium acetate-ethanol precipitation, following the instructions in the Sigma GenElute Plasmid Maxiprep Kit from yesterday. Final volume of the precipitation concentration will be 100 µL, a theoretical 5-fold increase in concentration. DNA QuantificationI ran each sample in duplicate, and at straight (undiluted) and 10x diluted concentrations.
There's a little bit of saturation of the instrument at concentrations around 2000 ng/µL, but overall the results look very consistent with each other and with expected values. Recovery from the precipitation is 85%.
|