Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/22

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qPCR Control Plate for mCherry & GAPD targets <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Overview

To establish some quality control standards for the RNA-seq and qPCR experiments, I performed qPCR on all dilutions of all templates using mCherry & GAPD as targets.

Templates to be used are:

  • ✓ U2OS (1:10, 1:100, 1:1000)
  • ✓ KAH126-U2OS (1:10, 1:100, 1:1000)
  • SK-N-SH (1:10, 1:100, 1:1000)

Also will be trying out a duplex reaction with GAPD & mCherry primers in the same sample, using a template that shows strong mCherry signal.

qPCR plate layout for U2OS & KAH126-U2OS templates
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