Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/22: Difference between revisions
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| TRUE||G1/G4||U2OSKAH126_C001 1:10||mCherry||GAPD||23.51659697||19.4546205||5.99E-02|| | | TRUE||G1/G4||U2OSKAH126_C001 1:10||mCherry||GAPD||23.51659697||19.4546205||5.99E-02|| | ||
|- | |- | ||
| TRUE||G7/G10||U2OSKAH126_C001 1:100||mCherry||GAPD||26.19546455||22.47707261||7.60E-02 | | TRUE||G7/G10||U2OSKAH126_C001 1:100||mCherry||GAPD||26.19546455||22.47707261||7.60E-02|| | ||
|- | |- | ||
| TRUE||H1/H4||U2OSKAH126_C001 1:1000||mCherry||GAPD||28.40332046||26.65931589||0.2985 | | TRUE||H1/H4||U2OSKAH126_C001 1:1000||mCherry||GAPD||28.40332046||26.65931589||0.2985|| | ||
|} | |} | ||
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Revision as of 15:35, 23 April 2015
qPCR Control Plate for mCherry & GAPD targets | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewTo establish some quality control standards for the RNA-seq and qPCR experiments, I performed qPCR on all dilutions of all templates using mCherry & GAPD as targets. Templates to be used are:
Also will be trying out a duplex reaction with GAPD & mCherry primers in the same sample, using a template that shows strong mCherry signal. qPCR plate layout for U2OS & KAH126-U2OS templatesqPCR results for U2OS & KAH126-U2OS templatesExperiment: DualColorProbe_UPL_DNyer042215 Active filters: FAM (465-510), VIC / HEX / Yellow555 (533-580)
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